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An extension reaction of an omega primer requires hybridization of both 5p arm and 3p arm.In a typical design, the binding energy of the 5p arm to a template is the same as that of the priming segment of a regular primer. cctrl=public,max-age=518400&quality=90&imagesource=IMLFOH&mark=1&watermark=0&width=422&height=315&filename=0957/1005957/1005957O1404889648.jpg" data-r-id="10" data-price-id="5" data-price="1.48" data-is-hh="True" data-is-online="true" data-bio-page="/live-sex-chat/cam-girls/Ur Cute Sarah" data-is-auto-play="false" data-is-room-full="False" //i0.wlmediahub.com/imagesrv/imp_getimage?cctrl=public,max-age=518400&quality=90&imagesource=IMLFOH&mark=1&watermark=0&width=422&height=315&filename=264347/264347O1173444298.jpg" data-r-id="10" data-price-id="5" data-price="3.8" data-is-hh="False" data-is-online="true" data-bio-page="/live-sex-chat/cam-girls/Blon Playmate" data-is-auto-play="false" data-is-room-full="False" //i0.wlmediahub.com/imagesrv/imp_getimage?Mismatches between 3p arm and a template destabilize the looped structure and cause the dissociation of the 3p arm from the template and reduces the probability of polymerase extension of the primer.Additionally, the 3p arm is short and by itself does not provide a sufficient inter-molecular binding with a template and does not sustain any significant polymerase extension reactions.If you are happy to be contacted by a Daily Trust journalist please leave a telephone number that we can contact you on.

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