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Selector .selector_input_interaction .selector_input. Selector .selector_input_interaction .selector_spinner. I will usually poke my head in there at least once a year - usually encountering at least one old friend from days gone by.The vesicular acetylcholine transporter gene (Slc18a3 or VACh T) within the Chat locus on the BAC transgene was not disrupted.The donating investigator reports that these Ch AT-mh Ch R2-YFP line 5 mice are expected to have a similar VACh T overexpression phenotype as reported for Ch AT-mh Ch R2-YFP line 6 (Stock No. That is, VACh T overexpression in hippocampus and brainstem results in increased cholinergic tone; the functional consequence of which is increased physical endurance but severe cognitive deficits in attention, working memory and spatial memory.

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As such, illuminating mh Ch R2-expressing neurons with blue light (450-490 nm) leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells.

The donating investigator reports that EYFP expression is only weakly visible by direct fluorescence (epifluorescence microscope), but can be amplified by antibody enhancement in fixed brain sections.

Mice hemizygous for the Ch AT-Ch R2-YFP BAC transgene are viable and fertile (see note below), with expression of the mh Ch R2:: YFP fusion protein directed to cholinergic neuronal populations by the mouse choline acetyltransferase (Chat or Ch AT) promoter/enhancer regions on the BAC transgene.

The mh Ch R2:: YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mh Ch R2; also called h Ch R2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP).

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